A method for cryogenic preservation of human biopsy specimens and subsequent organoid culture
YH Tsai, M Czerwinski, A Wu, MK Dame… - Cellular and …, 2018 - cmghjournal.org
Results/Discussion Endoscopic biopsy specimens were collected with 8-mm2 biopsy
forceps (average biopsy size, 5 mm2), transported to the laboratory, divided further into 2-to
3-mm2 fragments, then transferred into a cryopreservation vial containing freezing medium.
We tested 2 freezing media formulations: a complex medium (LWRN, fetal bovine serum,
dimethyl sulfoxide, Y27632, CHIR99021; see Supplementary Materials and Methods
section) 7, 9; and a simple freezing medium (Dulbecco's modified Eagle medium/F12, 10 …
forceps (average biopsy size, 5 mm2), transported to the laboratory, divided further into 2-to
3-mm2 fragments, then transferred into a cryopreservation vial containing freezing medium.
We tested 2 freezing media formulations: a complex medium (LWRN, fetal bovine serum,
dimethyl sulfoxide, Y27632, CHIR99021; see Supplementary Materials and Methods
section) 7, 9; and a simple freezing medium (Dulbecco's modified Eagle medium/F12, 10 …
